Journal: ACS Materials Au

Article Title: 3D-Printed Biohybrid PE/PCL Biphasic Osteochondral Plug for Knee Cartilage Repair

doi: 10.1021/acsmaterialsau.5c00193

Figure Lengend Snippet: CHON-001 ATCC test on PCL–RGD and PCL–HA samples compounded in PCL (Resomer C212, Evonik Industries AG). Cell proliferation assay was performed in human chondrocytes seeded onto PCL-based materials. Fold change in chondrocyte numbers was assessed in each biomaterial compared to control (PCL) by Kruskal wallis test whereby * is p value <0.05. Representative data with 4 technical replicates per group.

Article Snippet: Human chondrocytes (CHON-001) were purchased from ATCC (Cat No: CRL-2846) and maintained in complete media (Dulbecco’s Modified Eagle’s Medium supplemented with 10% Fetal Bovine Serum and 0.1 mg/mL G418) and incubated at 37 °C with 95% Air and 5% CO 2 as per ATCC recommendation.

Techniques: Proliferation Assay, Control

Journal: ACS Materials Au

Article Title: 3D-Printed Biohybrid PE/PCL Biphasic Osteochondral Plug for Knee Cartilage Repair

doi: 10.1021/acsmaterialsau.5c00193

Figure Lengend Snippet: Porcine knee joint at 24 weeks with OC plug implanted (a), extracted sample with 68% closure measured using Fiji software (b), porcine knee joint with microfracture (c). H&E staining images for corresponding knee joints where (d) cartilage with significant viable chondrocytes are observed in sample with plug embedded (white space is the OCP) and (e) Little cartilage growth in sample with microfracture.

Article Snippet: Human chondrocytes (CHON-001) were purchased from ATCC (Cat No: CRL-2846) and maintained in complete media (Dulbecco’s Modified Eagle’s Medium supplemented with 10% Fetal Bovine Serum and 0.1 mg/mL G418) and incubated at 37 °C with 95% Air and 5% CO 2 as per ATCC recommendation.

Techniques: Software, Staining

Journal: bioRxiv

Article Title: Characterization of emerging Oropouche virus tropism and pathogenicity

doi: 10.64898/2026.03.25.714204

Figure Lengend Snippet: A: dPCR results of human chondrocytes (HC, left), Fibroblast-Like Synoviocytes (HFLS, middle) and muscular cells (CHQ; right) cultures supernatants infected with OROV at MOI 0.1 and collected at different times (24, 48, 72 h p.i.). The graphs show 18 points (except HFLS with 12 points) per condition, as well as the mean and geometric standard deviation. ns: not significant; *: p-value < 0.05; **: p-value < 0.01; ***: p-value < 0.001; ****: p-value < 0.0001 (Dunn-Bonferroni non-parametric test). B: HC (left), HFLS (middle), and CHQ (right) cells were infected with OROV at different MOIs. Cell lysates were collected at 48 or 72h p.i. and analysed by Western blot, using antibodies against calnexin (CNX) as internal control and against the N protein of OROV (OROV N). C: The supernatants from HC (left), HFLS (middle) and CHQ (right) cultures were collected at different times after infection with OROV at MOI 0.1. The viral titer was determined on Vero cells using xCELLigence technology from a standard range. Each condition has 6 replicates. ns: not significant; *: p-value < 0.05; **: p-value < 0.01 (Dunn-Bonferroni non-parametric test).

Article Snippet: Human chondrocytes (HC) and synoviocytes (HFLS) were obtained from Cell Applications, (Ref PB-402-05a and 408K-05a, respectively) and cultured according to manufacturer’s instructions.

Techniques: Infection, Standard Deviation, Western Blot, Control

Journal: bioRxiv

Article Title: Characterization of emerging Oropouche virus tropism and pathogenicity

doi: 10.64898/2026.03.25.714204

Figure Lengend Snippet: HC, HFLS and CHQ cells were infected on coverslips with OROV at MOI 0.1. Gc protein of the OROV virus was detected (green), and nuclei were also labelled (blue). The scale bar represents 200 µm.

Article Snippet: Human chondrocytes (HC) and synoviocytes (HFLS) were obtained from Cell Applications, (Ref PB-402-05a and 408K-05a, respectively) and cultured according to manufacturer’s instructions.

Techniques: Infection, Virus

Journal: Journal of Biological Chemistry

Article Title: Transcriptional Repression of Matrix Metalloproteinase Gene Expression by the Orphan Nuclear Receptor NURR1 in Cartilage

doi: 10.1074/jbc.m608327200

Figure Lengend Snippet: FIGURE2.PGE2isapotentinducerofNURR1inchondrocytes.A,primaryhumanchondrocytesderivedfrom patients with OA were treated with PGE2 (10 M) for the times indicated. Nur77, NURR1, and NOR-1 mRNAs were measured by real-time RT-PCR, and levels were normalized to GAPDH. Results are representative of expression profiles from chondrocytes derived from five different patients. B, SW1353 cells were treated in triplicate with PGE2 (1 M) for the times indicated (hours). Nur77, NURR1, and NOR-1 mRNAs were measured by real-time RT-PCR, and levels were normalized to GAPDH. C, nuclear extracts were generated from SW1353 cells treated with PGE2 (1 M) for the times indicated. Western blotting was conducted with anti-NURR1 polyclonal antibody. In vitro translated NURR1 is shown as a positive control. Untr, untreated.

Article Snippet: Human chondrocytes were maintained in chondrocyte growth medium (Cell Applications) as monolayer cultures for not more than 3 days to maintain the chondrocyte phenotype.

Techniques: Quantitative RT-PCR, Expressing, Derivative Assay, Generated, Western Blot, In Vitro, Positive Control

Journal: Arthritis Research & Therapy

Article Title: Randomized controlled studies on the efficacy of antiarthritic agents in inhibiting cartilage degeneration and pain associated with progression of osteoarthritis in the rat

doi: 10.1186/s13075-016-0921-5

Figure Lengend Snippet: Efficacy of drugs in NF-κB, MMP-13, and bone remodeling assays

Article Snippet: The human chondrocytes used in toxicity and MMP studies were procured from Lonza in accordance with U.S. Food and Drug Administration regulations (21 CFR part 1271: Human Cells, Tissues, and Cellular and Tissue-Based Products) that govern tissue banking.

Techniques: In Vivo, Control, Activation Assay